首都医学科学创新中心-贾立加

平台主任

研究人员

贾立加

jial(at)cimrbj.ac.cn

冷冻电镜平台主任

jial(at)cimrbj.ac.cn

河北农业大学遗传学学士

中国农业大学数量遗传学硕士

中国科学院遗传与发育生物学研究所结构生物学博士

工作经历

2025.02至今

首都医学科学创新中心冷冻电镜平台平台主任

2022.01-2025.01

德克萨斯大学圣安东尼奥健康科学中心（UTHSCSA）助理教授/研究系列，冷冻电镜平台技术主任

2021.01-2021.12

德克萨斯大学圣安东尼奥健康科学中心（UTHSCSA）博士后研究员

2019.09-2020.12

佛罗里达州立大学博士后研究员

2017.07-2019.09

中国科学院生物物理研究所博士后研究员

平台介绍

冷冻电镜平台是首都医学科学创新中心技术支撑体系的重要组成部分，致力于提供基于冷冻电子显微成像技术的专业服务。包括大分子复合物的高分辨率结构解析、复杂细胞与亚细胞超微结构观察、免疫胶体金精准定位以及原位结构生物学等。平台提供从样品生化质量评估、负染筛选与优化、玻璃化冷冻制样、自动化高通量数据采集，到三维结构重构与解析的全流程技术服务。针对不同类型的研究对象提供定制化实验策略与技术方案，显著提高冷冻电镜结构解析的成功率、稳定性与研究效率。通过玻璃化冷冻技术与高分辨电子显微成像等手段，为揭示蛋白质功能机制、细胞动态过程及分子调控网络提供关键结构证据。为新型靶向药物设计、疫苗研发以及精准医学研究提供关键技术支撑。平台积极与用户合作，开发新的结构解析方法。

代表性文章 *：共同第一作者; #：共同通讯作者

Nayak D, Jia L, Bury PDS, Ruben EA, Shukla A, Nayak A, Stratton CM, Ebadi P, Cho H, Tumanova AA, Varughese JT, Yuan L, Gao F, Cano KE, Davies C, Sung P, Gack MU, Wasmuth EV, Olsen SK. Cryo-EM structures of UBA6 reveal mechanisms of E1–E2 specificity and dual FAT10/ubiquitin thioester transfer. Nat Commun, 2026. DOI: 10.1038/s41467-026-69882-3

Nayak A*, Nayak D*, Jia L*, Ruben EA, Viswanadhapalli S, Dos Santos Bury P, Nassar KM, Yu CH, Tumanova AA, Stratton CM, Ebadi P, Ivanov DN, Sung P, Vadlamudi RK, Wasmuth EV, Olsen SK. Cryo-EM structures reveal the molecular mechanism of SUMO E1-E2 thioester transfer. Nat Struct Mol Biol, 2025, 32: 2441-2453. DOI: 10.1038/s41594-025-01681-8

Jia L, Ruben EA, Suarez HJ, Olsen SK, Wasmuth EV. Single particle cryo-electron microscopy with an enhanced 200 kV cryo-TEM configuration achieves near-atomic resolution. bioRxiv, 2024. DOI: 10.1101/2024.05.07.593029

Afsar M, Liu G, Jia L, Ruben EA, Nayak D, Sayyad Z, Bury PDS, Cano KE, Nayak A, Zhao X, Shukla A, Sung P, Wasmuth EV, Gack MU, Olsen SK. Cryo-EM structures of Uba7 reveal the molecular basis for ISG15 activation and E1-E2 thioester transfer. Nat Commun, 2023, 14: 4786. DOI: 10.1038/s41467-023-39780-z

Rawal Y*, Jia L*, Meir A*, Zhou S, Kaur H, Ruben EA, Kwon Y, Bernstein KA, Jasin M, Taylor AB, Burma S, Hromas R, Mazin AV, Zhao W, Zhou D, Wasmuth EV, Greene EC, Sung P, Olsen SK. Structural insights into BCDX2 complex function in homologous recombination. Nature, 2023, 619: 640-649. DOI: 10.1038/s41586-023-06219-w

Jia L, Rawal Y, Meir A, Wasmuth EV, Greene EC, Sung P, Olsen SK. Structural and functional insights into the role of human BCDX2 complex in RAD51-dependent homologous recombination by cryoEM. J Biol Chem, 2023, 299: S642. DOI: 10.1016/j.jbc.2023.104187

Yuan L, Gao F, Lv Z, Nayak D, Nayak A, Bury PDS, Cano KE, Jia L, Atligan FC, Oleinik N, Ogretmen B, El Oualid F, Wasmuth EV, Olsen SK. Crystal structures reveal catalytic and regulatory mechanisms of the dual-specificity ubiquitin/FAT10 E1 enzyme Uba6. Nat Commun, 2022, 13: 4880. DOI: 10.1038/s41467-022-32613-5

Lv Z, Cano KE, Jia L, Drag M, Huang TT, Olsen SK. Targeting SARS-CoV-2 proteases for COVID-19 antiviral development. Front Chem, 2022, 9: 819165. DOI: 10.3389/fchem.2021.819165

查看全部文章请点击：这里

学术著作

Jia L. Running a Cost-Effective Modern Cryo-EM Facility for SPA analysis. In: CRYO-ELECTRON MICROSCOPY IN STRUCTURAL BIOLOGY. Taylor & Francis Publishing Group of CRC Press, 2024. eBook ISBN: 9781003326106.

收录的冷冻电镜结构EMD

Cryo-EM structures of SUMO E1-E2 thioester transfer complexes. EMD-47110, EMD-47127 Jia L, Nayak D, Ruben EA, Nayak A, Wasmuth EV, Olsen SK

Cryo-EM structures of the human BCDX2 and BCDX2/ssDNA complexes. EMD-28961, EMD-29917 Jia L, Wasmuth EV, Ruben EA, Sung P, Rawal Y, Greene EC, Meir A, Olsen SK

Cryo-EM structures of human UBA7-UBE2L6-ISG15 thioester mimetic and adenylate complexes. EMD-40799, EMD-40407, EMD-40408, EMD-40409, EMD-40782 Afsar M, Jia L, Ruben EA, Olsen SK

Cryo-EM structures of human BCDX2 complex (Glacios/Selectris optimization series). EMD-44757, EMD-44758, EMD-44759 Jia L, Ruben EA, Olsen SK, Wasmuth EV

Cryo-EM structure of Human Nucleosome collected by EPU on Glacios at 3.3 Angstrom resolution. EMD-44470 Jia L, Ruben EA, Olsen SK, Wasmuth EV

Cryo-EM structure of Aldolase collected by EPU on Glacios at 2.6 Angstrom resolution. EMD-41669 Jia L, Ruben EA, Olsen SK, Wasmuth EV

Cryo-EM structure of Apoferritin collected by Leginon on Glacios at 2.1 Angstrom resolution. EMD-41622 Jia L, Ruben EA, Olsen SK, Wasmuth EV